Primer

Part:BBa_K5427067:Design

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


RV_Pet22_KerDZ


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 49
    Illegal XbaI site found at 34
    Illegal XbaI site found at 91
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 49
    Illegal NotI site found at 41
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 49
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 49
    Illegal XbaI site found at 34
    Illegal XbaI site found at 91
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 49
    Illegal XbaI site found at 34
    Illegal XbaI site found at 91
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Reverse primer for linearization of pET22b+ with KerDZ.


Source

Not applicable.

References